Optimization of a mouse immunization protocol with Paracoccidioides brasiliensis antigens
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چکیده
منابع مشابه
Characterization of the cellular antigens of Paracoccidioides brasiliensis yeast form.
Antigenic components of the yeast extract of Paracoccidioides brasiliensis Linder 2511 cultured for 3, 8, 20, 30, and 60 days were examined by the Western blot (immunoblot) technique. The 3-day extract was chosen for characterization of the antigenic components because its stability did not vary with time and it contained all antigens identified by patient sera. Antibodies to cross-reacting ant...
متن کاملHuman serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate Reatividade de anticorpos de soros humanos a antígenos de Paracoccidioides brasiliensis tratados com meta-periodato
In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of para...
متن کاملImmunological response to cell-free antigens of Paracoccidioides brasiliensis: relationship with clinical forms of paracoccidioidomycosis.
Sera from patients with the acute (AF) and chronic (CF) forms of paracoccidioidomycosis (PCM) were tested against Paracoccidioides brasiliensis cell-free antigens by Western blot (immunoblot). The CFA preparation contained components ranging in molecular mass from 18 to 102 kDa. The immunoglobulin G (IgG) reactivity profiles were similar for patients with both forms of the disease, and the 43-k...
متن کاملHuman serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate Reatividade de anticorpos de soros humanos a antígenos de Paracoccidioides brasiliensis tratados com metaperiodato sódico
In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of para...
متن کاملParacoccidioides brasiliensis AND Paracoccidioides lutzii, A SECRET LOVE AFFAIR
To commemorate Prof. Carlos da Silva Lacaz's centennial anniversary, the authors have written a brief account of a few, out of hundreds, biological, ecological, molecular and phylogenetic studies that led to the arrival of Paracoccidioides lutzii, hidden for more than a century within Paracoccidioides brasiliensis. Lacaz's permanent interest in this fungus, and particularly his conviction on th...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 1995
ISSN: 0074-0276
DOI: 10.1590/s0074-02761995000100011